Activity-based NEK4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
NEK4
Enzyme
Human NEK4
HGNC
NEK4
Synonym
NRK2, STK2
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 277 |
| Ro 31-8220 | 79,000 |
| GW 5074 | 13,200 |
| Rottlerin | 16,800 |
Scientific Information
P51957 (http://www.uniprot.org/uniprot/P51957)
Screening Location
Malvern, USA
Reference compound IC50 for NEK4
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