Activity-based NEK5 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
NEK5
Enzyme
Human NEK5, wt
HGNC
NEK5
Manning ID
NEK5
Synonym
MGC75495
UniProt
Q6P3R8 (NEK5_HUMAN)
Kinase Type
Protein kinase
Kinase Group
Other
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-GSK3, ATP
Reaction
Substrate + 33P-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 700 |
| Sorafenib | >100000 |
| Sunitinib | >100000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | NEK5 |
| wt Profiler Panel | Yes |
| IC50 wt Profiler Panel | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – NEK5 (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 2121-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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