NEK5 Kinase Assay Service

Activity-based NEK5 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
NEK5
Enzyme
Human NEK5
HGNC
NEK5
Synonym
MGC75495
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine42
Ro 31-822018,000
GW507428,000
H-89>50,000
Rottlerin600
Scientific Information
Q6P3R8 (http://www.uniprot.org/uniprot/Q6P3R8)
Screening Location
Malvern, USA
Reference compound IC50 for NEK5
Reference compound IC50 for NEK5