NEK5 Kinase Assay Service

Activity-based NEK5 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
NEK5

Enzyme
Human NEK5

HGNC
NEK5

Synonym
MGC75495

Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 42
Ro 31-8220 18,000
GW5074 28,000
H-89 >50,000
Rottlerin 600
Scientific Information
Q6P3R8 (http://www.uniprot.org/uniprot/Q6P3R8)

Screening Location
Malvern, USA

Reference compound IC50 for NEK5
Reference compound IC50 for NEK5

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