NEK6 NanoBRET Kinase Assay

NEK6 NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
Target Overview

Target

NEK6

HGNC Symbol

NEK6

Synonyms

SID6-1512

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties

Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently expressing NanoLuc- NEK6 Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-9 and then treated with reference compound CC1 pan-kinase inhibitor for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal dose-response equation.

Readout

Binding of compound to target kinase

Reference Compound IC50s
Compounds IC50 (M)
CC1 pan-Kinase Inhibitor 4.560e-007

Screening Location

Malvern, USA

Further information

More information can be found on our website NanoBRET Target Engagement Intracellular Kinase Assay Services.

Reference Compound IC50 for NEK6