NEK7 Kinase Assay Service

Activity-based NEK7 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
NEK7

Enzyme
Human NEK7

HGNC
NEK7

Synonym
NEK7

Substrates
Casein, 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 33,000
Ro 31-8220 >100,000
GW 5074 47,000
H-89 >100,000
PKR Inhibitor 11,000
Scientific Information
Q8TDX7 (http://www.uniprot.org/uniprot/Q8TDX7)

Screening Location
Malvern, USA

Reference compound IC50 for NEK7
Reference compound IC50 for NEK7