NEK8 Kinase Assay Service

Activity-based NEK8 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
NEK8

Enzyme
Human NEK8

HGNC
NEK8

Synonym
NEK8

Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 23
Ro 31-8220 10,000
GW 5074 90,000
H-89 >100,000
Rottlerin >100,000
Scientific Information
Q86SG6 (http://www.uniprot.org/uniprot/Q86SG6)

Screening Location
Malvern, USA

Reference compound IC50 for NEK8
Reference compound IC50 for NEK8