Activity-based NIK kinase assay for compound screening and profiling via radiometric ADP-Glo kinase assay.
Target Overview
CAT#
NIK (ADP-Glo)
Enzyme
Human NIK
HGNC
MAP3K14
Synonym
Mitogen-activated protein kinase kinase kinase 14, NF-kappa-beta-inducing kinase, Serine/threonine-protein kinase NIK
Assay Properties
Substrates
ATP, 1 µM or 1 mM
Reaction
ATP = ADP + Pi
Readout
ADP-Glo Detection
Reference Compound IC50s
| Compounds | IC50 |
|---|---|
| B022 (10 µM ATP ) | 2.2 nM |
| NIK SMI (10 µM ATP) | 2.1 nM |
| Staurosporine (10 µM ATP) | 18 nM |
| Staurosporine (1 mM ATP) | 1.2 µM |
Scientific Information
Q99558 (http://www.uniprot.org/uniprot/Q99558)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Screening Assay Services
Reference compound inhibition for NIK
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