NIK/MAP3K14 Kinase Assay Service

Activity-based NIK/MAP3K14 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
NIK/MAP3K14

Enzyme
Human NIK/MAP3K14

HGNC
MAP3K14

Synonym
NIK, FTDCR1B, HS, HSNIK

Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 1 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 13.4
Ro 31-8220 27,400
GW 5074 2,100
Scientific Information
Q99558 (http://www.uniprot.org/uniprot/Q99558)

Screening Location
Malvern, USA

Reference compound IC50 for NIK
Reference compound IC50 for NIK

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