Activity-based NIM1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
NIM1
Enzyme
Human NIM1
HGNC
NIM1K
Synonym
NIM1
Substrates
Peptide substrate, [KKKVSRSGLYRSPSMPENLNRPR], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 110 |
| Ro 31-8220 | 22,400 |
| GW5074 | 14,200 |
| H-89 | >50,000 |
| Rottlerin | >50,000 |
Scientific Information
Q8IY84 (http://www.uniprot.org/uniprot/Q8IY84)
Screening Location
Malvern, USA
Reference compound IC50 for NIM1
PDF