NQO1 NAD(P)H Dehydrogenase Assay Service

NQO1 NAD(P)H Dehydrogenase Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
CAT#

NQO1

Enzyme

Human NQO1

Substrate

5 µM resazurin, 20 µM NADH

Reaction

Resazurin + NADH = Resorufin + NAD

Resorufin fluorescence, kinetic measurement

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Dicoumarol 5.6

 

Scientific Information

Reference compound inhibition of NQO1

NQO ic50 determination