NRMT1 Methyltransferase Assay Service

Activity-based NRMT1 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
NRMT1
Enzyme
Human NRMT1
Synonym
N-terminal Xaa-Pro-Lys N-methyltransferase 1, NTMT1, N-terminal RCC1 methyltransferase, Methyltransferase-like protein 11A, METTL11A
Substrates
RCC1, 5 µMSAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + N-terminal-A/S/PPK-protein = S-adenosyl-L-homocysteine + protein [methyl-³H]-L- lysine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
SAH3,300
Chaetocin>100,000
Sinefungin6,900
Suramin>100,000
Scientific Information
Q9BV86 (http://www.uniprot.org/uniprot/Q9BV86)
Screening Location
Malvern, USA
Reference compound IC50 for NRMT1
Reference compound IC50 for NRMT1