NRMT2 Methyltransferase Assay Service

Activity-based NRMT2 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
NRMT2
Enzyme
Human NRMT2
Synonym
Alpha N-terminal protein methyltransferase 1B, X-Pro-Lys N-terminal protein methyltransferase 1B, NTM1B, Methyltransferase-like protein 11B, METTL11B
Substrates
RCC1, 5 µMSAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + N-terminal-A/S/PPK-protein = S-adenosyl-L-homocysteine + protein [methyl-³H]-L- lysine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
SAH2,500
Chaetocin>100,000
Sinefungin4,700
Suramin>100,000
Scientific Information
Q5VVY1 (http://www.uniprot.org/uniprot/Q5VVY1)
Screening Location
Malvern, USA
Reference compound IC50 for NRMT2
Reference compound IC50 for NRMT2