NSD1 Methyltransferase Assay Service

Activity-based NSD1 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
NSD1
Enzyme
Human NSD1
Synonym
Probable histone-lysine N-methyltransferase H3 lysine-36 and H4 lysine-20 specific, Androgen receptor coactivator 267 kDa protein, Androgen receptor-associated protein of 267 kDa, H3-K36-HMTase, H4-K20-HMTase, Lysine N-methyltransferase 3B, Nuclear receptor-binding SET domain-containing protein 1, ARA267, KMT3B
Substrates
Nucleosomes, 0.05 mg/mlSAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L- lysine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- lysine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
SAH45,000
Sinefugin110,000
BIX01294>200,000
Chaetocin2,300
Scientific Information
Q96L73 (http://www.uniprot.org/uniprot/O96L73)
Screening Location
Malvern, USA
Reference compound IC50 for NSD1
Reference compound IC50 for NSD1