Activity-based NSD1 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Probable histone-lysine N-methyltransferase H3 lysine-36 and H4 lysine-20 specific, Androgen receptor coactivator 267 kDa protein, Androgen receptor-associated protein of 267 kDa, H3-K36-HMTase, H4-K20-HMTase, Lysine N-methyltransferase 3B, Nuclear receptor-binding SET domain-containing protein 1, ARA267, KMT3B
Nucleosomes, 0.05 mg/mlSAM, 1 µM
S-adenosyl-L-[methyl-³H]methionine + histone L- lysine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- lysine
Reference Compound IC50s
Reference compound IC50 for NSD1