NSD3 Methyltransferase Assay Service

Activity-based NSD3 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
NSD3
Enzyme
Human NSD3
Synonym
Histone-lysine N-methyltransferase NSD3, Nuclear SET domain-containing protein 3 Protein whistle, WHSC1-like 1 isoform 9 with methyltransferase activity to lysine, Wolf-Hirschhorn syndrome candidate 1-like protein 1, WHSC1L1, DC28
Substrates
Nucleosomes, 0.05 mg/mlSAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L- lysine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- lysine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
SAH>100,000
Chaetocin192
Suramin320
Sinefugin>100,000
Scientific Information
Q9BZ95 (http://www.uniprot.org/uniprot/Q9BZ95)
Screening Location
Malvern, USA
Reference compound IC50 for NSD3
Reference compound IC50 for NSD3