Activity-based NSD3 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Histone-lysine N-methyltransferase NSD3, Nuclear SET domain-containing protein 3 Protein whistle, WHSC1-like 1 isoform 9 with methyltransferase activity to lysine, Wolf-Hirschhorn syndrome candidate 1-like protein 1, WHSC1L1, DC28
Nucleosomes, 0.05 mg/mlSAM, 1 µM
S-adenosyl-L-[methyl-³H]methionine + histone L- lysine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- lysine
Reference Compound IC50s
Reference compound IC50 for NSD3