Activity-based NSD3 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
NSD3
Enzyme
Human NSD3
Synonym
Histone-lysine N-methyltransferase NSD3, Nuclear SET domain-containing protein 3 Protein whistle, WHSC1-like 1 isoform 9 with methyltransferase activity to lysine, Wolf-Hirschhorn syndrome candidate 1-like protein 1, WHSC1L1, DC28
Substrates
Nucleosomes, 0.05 mg/ml
SAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L- lysine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- lysine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| SAH | >100,000 |
| Chaetocin | 192 |
| Suramin | 320 |
| Sinefugin | >100,000 |
Scientific Information
Q9BZ95 (http://www.uniprot.org/uniprot/Q9BZ95)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Methyltransferase Assays.
Reference compound IC50 for NSD3
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