OPRK1 cAMP Antagonist Assay for compound screening and profiling.
Target Information
Cell Catalog#
LLC-2054
GPCR Name
Human Kappa-type opioid receptor
Gene Name
OPRK1
GPCR Sub Family
Opioid receptors
Coupling Partner
Gαi
Uniprot Number
P41145 (https://www.uniprot.org/uniprotkb/P41145/entry)
Cell Type
HEK293/OPRK1/β-arrestin-2 stable cell line
Assay Information
Assay Principle
The cAMP antagonist assay detects antagonist-mediated promotion of cAMP production via OPRK1 activation in HEK293/OPRK1/β-arrestin-2 stable cell line.
Assay Type
Cell-based functional assay
Functional Mode
Antagonist
Assay Readout
FRET fluorescence
Reference Compound EC50
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website GPCR Assay and Profiling Services
Representative dose–response curve of reference antagonist for OPRK1
