p300 Histone Acetyltransferase Assay Service

Activity-based p300 Histone Acetyltransferase Assay for compound screening and profiling via radiometric HAT assay.
CAT#
p300
Enzyme
Human p300
Synonyms
Histone acetyltransferase p300, E1A-associated protein p300, EP300
Substrates
Histone H3, 5 μM
Acetyl CoA, 3 μM
Reaction
[Acetyl-³H]-Acetyl Coenzyme A + histone L-lysine = Coenzyme A + [Acety-³H]-L-lysine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Anacardic Acid35,000
Garcinol26,000
Curcumin34,000
C646200
Scientific Information
Q09472 (http://www.uniprot.org/uniprot/Q09472)
Screening Location
Malvern, USA
Reference Compound IC50 for p300
Reference Compound IC50 for p300