p300 Histone Acetyltransferase Assay Service

Activity-based p300 Histone Acetyltransferase Assay for compound screening and profiling via radiometric HAT assay.

Target Overview

CAT#

p300

Enzyme

Human p300

Synonyms

Histone acetyltransferase p300, E1A-associated protein p300, EP300

Substrates

Histone H3, 5 μM
Acetyl CoA, 0.5 μM

Assay Properties

Reaction

[Acetyl-³H]-Acetyl Coenzyme A + histone L-lysine = Coenzyme A + [Acety-³H]-L-lysine

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Anacardic Acid 35,000
Garcinol 26,000
Curcumin 34,000
C646 200

Scientific Information

Q09472 (http://www.uniprot.org/uniprot/Q09472)

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Histone Acetyltransferase Assay (HAT) Services for Drug Discovery

Reference Compound IC50 for p300

Reference Compound IC50 for p300