p38a (T106M) Kinase Assay Service

Activity-based p38a (T106M) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
P38α (T106M)

Enzyme
Human P38α (T106M)

HGNC
MAPK14 (T106M)

Synonym
p38-alpha, CSBP, CSBP1,

Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 12,400
Ro 31-8220 50,000
GW 5074 18,900
Scientific Information
Q16539 (http://www.uniprot.org/uniprot/Q16539)

Screening Location
Malvern, USA

Reference compound IC50 for P38α (T106M)
Reference compound IC50 for P38α (T106M)

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