p53/MDM2 NanoBRET Cellular Protein Interaction Assay

NanoBRET Cellular p53/MDM2 Protein Interaction Assay

Target Overview


Cell Line

Human embryonic kidney HEK293 cell line from ATCC

Assay Properties
Assay Format

NanoBRET assay

Assay Protocol

HEK-293 cells co-expressing p53-HaloTag and MDM2-NanoLuc vectors were plated in 384-well plate and pre-treated with/without HaloTag NanoBRET 618 Ligand. The cells were then treated with various concentrations of reference compound Nutlin-3 for 22 hours before addition of Nano-Glo Luciferase Assay Substrate and NanoBRET measurement on an Envision 2104 Multilabel Reader. IC50 curve was plotted and IC50 value was calculated using the GraphPad Prism program.

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Apoptosis Pathway Assay Services.

p53/MDM2 Interaction

p53/MDM2 Interaction