P70S6K/RPS6KB1 Kinase Assay Service

Activity-based P70S6K/RPS6KB1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
p70S6K/RPS6KB1

Enzyme
Human p70S6K/RPS6KB1

HGNC
RPS6KB1

Synonym
S6K, STK14A, P70S6K

Substrates
Peptide substrate, [KKRNRTLTK], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.9
Ro 31-8220 2.8
GW 5074 5,100
H-89 140
Rottlerin 37,000
Scientific Information
P23443 (http://www.uniprot.org/uniprot/P23443)

Screening Location
Malvern, USA

Reference compound IC50 for p70S6K/RPS6KB1
Reference compound IC50 for p70S6K/RPS6KB1

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