PAK1 Kinase Assay Service

Activity-based PAK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
PAK1

Enzyme
Human PAK1

HGNC
PAK1

Synonym
PAKalpha

Substrates
Peptide substrate, [RRRLSFAEPG], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.3
Ro 31-8220 1,800
GW 5074 >100,000
H-89 30,000
Rottlerin >100,000
Scientific Information
Q13153 (http://www.uniprot.org/uniprot/Q13153)

Screening Location
Malvern, USA

Reference compound IC50 for PAK1
Reference compound IC50 for PAK1