PAK2 Kinase Assay Service

Activity-based PAK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
PAK2

Enzyme
Human PAK2

HGNC
PAK2

Synonym
PAK65, PAKgamma

Substrates
Peptide substrate, [RRRLSFAEPG], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 2.6
Ro 31-8220 6,800
GW5074 35,000
H-89 39,000
Rottlerin >100,000
Scientific Information
Q13177 (http://www.uniprot.org/uniprot/Q13177)

Screening Location
Malvern, USA

Reference compound IC50 for PAK2
Reference compound IC50 for PAK2