PAK2 (Y443N) Kinase Assay Service

Activity-based PAK2 (Y443N) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview
CAT#

PAK2 (Y443N)

Enzyme

Human PAK2 (Y443N)

HGNC

PAK2 (Y443N)

Synonym

Serine/threonine-protein kinase PAK 2, p21-activated kinase 2, PAK-2, PAK65, Gamma-PAK, S6/H4 kinase

Substrates

Peptide substrate, [RRRLSFAEPG], 20 µM
ATP 10 µM

Assay Properties
Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 7.1
Ro 31-8220 11,500
GW5074 >100,000
H-89 >100,000
Rottlerin >100,000
Scientific Information

Q13177 (http://www.uniprot.org/uniprot/Q13177)

Screening Location

Malvern, USA

Reference compound IC50 for PAK2 (Y443N)