Activity-based PAK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PAK3
Enzyme
Human PAK3
HGNC
PAK3
Synonym
CDKN1A, MRX30, OPHN3, PAK3beta, bPAK
Substrates
Peptide substrate, [RRRLSFAEPG], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.4 |
| Ro 31-8220 | 5,300 |
| GW 5074 | >100,000 |
| H-89 | 28,000 |
| Rottlerin | >100,000 |
Scientific Information
O75914 (http://www.uniprot.org/uniprot/O75914)
Screening Location
Malvern, USA
Reference compound IC50 for PAK3
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