Activity-based PAK5 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PAK5
Enzyme
Human PAK5
HGNC
PAK5
Synonym
PAK7, PAK5, KIAA1264, MGC26232
Substrates
Peptide substrate, [RRRLSFAEPG], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 8.5 |
| Ro 31-8220 | 10,600 |
| GW 5074 | 12,000 |
| H-89 | >100,000 |
| Rottlerin | >100,000 |
Scientific Information
Q9P286 (http://www.uniprot.org/uniprot/Q9P286)
Screening Location
Malvern, USA
Reference compound IC50 for PAK5
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