PAK6 Kinase Assay Service

Activity-based PAK6 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PAK6
Enzyme
Human PAK6
HGNC
PAK6
Synonym
PAK6, PAK5
Substrates
Peptide substrate, [RRRLSFAEPG], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine2.9
Ro 31-822012,500
GW5074>100,000
H-8914,600
Rottlerin>100,000
Scientific Information
Q9NQU5 (http://www.uniprot.org/uniprot/Q9NQU5)
Screening Location
Malvern, USA
Reference compound IC50 for PAK6
Reference compound IC50 for PAK6