PAK6 NanoBRET Kinase Assay

PAK6 NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.

Target

PAK6

HGNC Symbol

PAK6

Synonyms

PAK6, PAK5

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently expressing NanoLuc-PAK6 Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-10 and then treated with reference compound Staurosporine for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal dose response equation.

Readout

Binding of compound to target kinase

Reference Compound IC50s
Compounds IC50 (M)
Staurosporine 1.134e-009
Screening Location

Malvern, USA

Reference compound IC50 for PAK6