Activity-based PASK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PASK
Enzyme
Human PASK
HGNC
PASK
Synonym
DKFZP434O051, DKFZp686P2031, KIAA0135, PASKIN, STK3
Substrates
Peptide substrate, [KKLNRTLSFAEPG], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 8.5 |
| Ro 31-8220 | 178 |
| GW 5074 | 400 |
| H-89 | 18,000 |
Scientific Information
Q96RG2 (http://www.uniprot.org/uniprot/Q96RG2)
Screening Location
Malvern, USA
Reference compound IC50 for PASK
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