Activity-based PBK/TOPK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PBK
Enzyme
Human PBK
HGNC
PBK
Synonym
TOPK, SPK, Nori-3, FLJ14385
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 141 |
| Ro 31-8220 | 22,900 |
| GW5074 | 153,000 |
| Rottlerin | 12,700 |
Scientific Information
Q96KB5 (http://www.uniprot.org/uniprot/Q96KB5)
Screening Location
Malvern, USA
Reference compound IC50 for PBK/TOPK
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