PDE7A Phoshodiesterase Assay Service

PDE7A Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#

PDE7A

Enzyme

Human PDE7A

Synonym

High affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A, HCP1, TM22

Substrate

0.2 µM cAMP

Assay Properties
Reaction

Nucleoside 3',5'-cyclic phosphate + H₂O = nucleoside 5'-phosphate

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
IBMX >100,000
Methoxymethyl-IBMX >100,000
Roliptam >100,000
BRL-50481 1,200
Scientific Information

Q13946 (http://www.uniprot.org/uniprot/Q13946)

Screening Location

Malvern, USA

Reference Compound IC50 for PDE7A

Reference Compound IC50 for PDE7A