PDGFRa-FIP1L1 Kinase Assay Service

Activity-based PDGFRa-FIP1L1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
-PDGFRa, FIP1L1
Enzyme
Human FIP1L1- PDGFRa fusion
HGNC
PDGFRA-FIP1L1
Synonym
PDGFR2, CD140a
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine5.8
PP2140
AG14782,500
PDGFR I-III35
D-644061,700
Scientific Information
FIP1L1: Q6UN15 (http://www.uniprot.org/uniprot/Q6UN15)PDGFRa: Q9DE49 (http://www.uniprot.org/uniprot/Q9DE49)
Screening Location
Malvern, USA
Reference compound IC50 for PDGFRa-FIP1L1
Reference compound IC50 for PDGFRa-FIP1L1