Activity-based PDGFRa(D842V) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PDGFRa(D842V)
Enzyme
Human PDGFRa(D842V)
HGNC
PDGFRa(D842V)
Synonym
PDGFR2, CD140a
Scientific Information
Q9DE49 (http://www.uniprot.org/uniprot/Q9DE49)
Assay Properties
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.42 |
| PP2 | 16.9 |
| AG1478 | 167 |
| D-64406 | 314 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Screening Assay Services.
Reference compound inhibition of PDGFRa(D842V)
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