Activity-based PDGFRa(G680R) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PDGFRα (G680R)
Enzyme
Human PDGFRα (G680R)
HGNC
PDGFRA (G680R)
Synonym
PGFRA, Alpha platelet-derived growth factor receptor
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 22 |
| PP2 | 23 |
| AG1478 | 27,700 |
| EGFR/ErbB Inhibitor | >100,000 |
| D-64406 | 8,600 |
Scientific Information
Q9DE49 (http://www.uniprot.org/uniprot/Q9DE49)
Screening Location
Malvern, USA
PDF