PDGFRalpha Kinase Activity Assay Service

Activity-based PDGFRalpha kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview

RBE Target ID

PDGFRalpha

Enzyme

Human PDGFRalpha, wt

HGNC

PDGFRA

Manning ID

PDGFRa

Synonym

PDGFR-alpha, CD140A, PDGFR2, PDGFRA, CD140a, PDGFR-alpha wt, PDGFRalpha, PDGFRa

UniProt

P16234 (PGFRA_HUMAN)

Kinase Type

Protein kinase

Kinase Group

TK

Assay Properties

Assay Format

33PanQinaseTM

Substrates

Poly(Ala,Glu,Lys,Tyr)6:2:5:1, ATP

Reaction

Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + 33P-ATP = 33P-Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 20
Sorafenib 300
Sunitinb 83

Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability

Screening Opportunities
FreeChoice PDGFRalpha
345 wt Profiler Yes
345 IC50 wt Profiler Yes
Mutant Profiler Addon No
Lipid Kinase Profiler Addon No
IC50 Profiler No
Recombinant Protein Availability
Active recombinant protein

Yes; ProQinaseTM – PDGFRalpha
(Activity verified by 33PanQinaseTM-Assay)

Product Number

1057-0000-1

Web Shop Reaction Biology Online Shop