PDK1 Kinase Activity Assay Service

Activity-based PDK1 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview

RBE Target ID

PDK1

Enzyme

Human PDK1, wt

HGNC

PDK1

Manning ID

PDK1

Synonym

PDPK1, PKB-like, PDK1/PDHK1, PDHK1, PDK1

UniProt

Q15118 (PDK1_HUMAN)

Kinase Type

Protein kinase

Kinase Group

Atypical

Assay Properties

Assay Format

33PanQinaseTM

Substrates

bio-4xCHKT, ATP

Reaction

bio-4xCHKT + 33P-ATP = 33P-bio-4xCHKT + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 11
Sorafenib 10000
Sunitinb 4900

Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability

Screening Opportunities
FreeChoice PDK1
345 wt Profiler Yes
345 IC50 wt Profiler Yes
Mutant Profiler Addon No
Lipid Kinase Profiler Addon No
IC50 Profiler No
Recombinant Protein Availability
Active recombinant protein

Yes; ProQinaseTM – PDK1
(Activity verified by 33PanQinaseTM-Assay)

Product Number

0367-0000-1

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