PDK1/PDHK1 Kinase Assay Service

Activity-based PDK1/PDHK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#

PDK1/PDHK1

Enzyme

Human PDK1/PDHK1

HGNC

PDK1

Synonym

PKB-like, PDHK1, Pyruvate dehydrogenase kinase isoform 1

Substrates

MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties
Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine >100,000
Ro 31-8220 >100,000
GW 5074 9,000
H-89 >100,000
Rottlerin >100,000
Scientific Information

Q15118 (http://www.uniprot.org/uniprot/Q15118)

Screening Location

Malvern, USA

Further information

More information can be found on our website Kinase Assays.

Reference compound IC50 for PDK1/PDHK1

Reference compound IC50 for PDK1/PDHK1