Activity-based PDK1/PDPK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PDK1/PDPK1
Enzyme
Human PDK1/PDPK1
HGNC
PDPK1
Synonym
PDK1
Substrates
Peptide substrate, [KTFCGTPEYLAPEVRREPRILSEEEQEMFRDFDYIADWC], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
| Staurosporine | 0.7 |
| Ro 31-8220 | 330 |
| GW 5074 | 6,000 |
| H-89 | >100,000 |
| Rottlerin | >100,000 |
Scientific Information
O15530 (http://www.uniprot.org/uniprot/O15530)
Screening Location
Malvern, USA
Further information
More information can be found on our website Kinase Assays.
Reference compound IC50 for PDK1/PDPK1
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