PDK2/PDHK2 Kinase Assay Service

Activity-based PDK2/PDHK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
PDK2/PDHK2

Enzyme
Human PDK2/PDHK2

HGNC
PDK2

Synonym
PDHK2

Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine >100,000
Ro 31-8220 >100,000
GW 5074 9,000
H-89 >100,000
Rottlerin >100,000
Scientific Information
Q15119 (http://www.uniprot.org/uniprot/Q15119)

Screening Location
Malvern, USA

Reference compound IC50 for PDK2/PDHK2
Reference compound IC50 for PDK2/PDHK2