Activity-based PDK2/PDHK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PDK2/PDHK2
Enzyme
Human PDK2/PDHK2
HGNC
PDK2
Synonym
PDHK2, Pyruvate dehydrogenase [lipoamide] kinase isozyme 2 mitochondrial, Pyruvate dehydrogenase kinase isoform 2
Scientific Information
Q15119 (http://www.uniprot.org/uniprot/Q15119)
Assay Properties
Substrates
PDHKtide + Mn, 40 µM
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | >100,000 |
| Ro 31-8220 | >100,000 |
| GW 5074 | 6,530 |
| H-89 | >100,000 |
| Rottlerin | >100,000 |
| VER-246608 | 52.7 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays.
Reference compound IC50 for PDK2/PDHK2
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