Activity-based PDK4/PDHK4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PDK4/PDKH4
Enzyme
Human PDK4/PDKH4
HGNC
PDK4
Synonym
PDHK4
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Ro 31-8220 | >100,000 |
| GW 5074 | 5,000 |
| Rottlerin | 47,600 |
Scientific Information
Q16654 (http://www.uniprot.org/uniprot/Q16654)
Screening Location
Malvern, USA
Reference compound IC50 for PDK4/PDKH4
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