PHKg1 Kinase Assay Service

Activity-based PHKg1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PHKγ1
Enzyme
Human PHKγ1
HGNC
PHKG1
Synonym
PHKg1
Substrates
Peptide substrate, [KKLNRTLSFAEPG], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine3.3
Ro 31-8220240
GW507411,000
H-8912,400
Scientific Information
Q16816 (http://www.uniprot.org/uniprot/Q16816)
Screening Location
Malvern, USA
Reference compound IC50 for PHKγ1
Reference compound IC50 for PHKγ1