Activity-based PHKg1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PHKγ1
Enzyme
Human PHKγ1
HGNC
PHKG1
Synonym
PHKg1
Substrates
Peptide substrate, [KKLNRTLSFAEPG], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 3.3 |
| Ro 31-8220 | 240 |
| GW5074 | 11,000 |
| H-89 | 12,400 |
Scientific Information
Q16816 (http://www.uniprot.org/uniprot/Q16816)
Screening Location
Malvern, USA
Reference compound IC50 for PHKγ1
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