PHKg2 Kinase Assay Service

Activity-based PHKg2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
PHKg2

Enzyme
Human PHKg2

HGNC
PHKG2

Synonym
PHKg2

Substrates
Peptide substrate, [KKLNRTLSFAEPG], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.4
Ro 31-8220 22,000
GW 5074 9,400
H-89 8,900
Scientific Information
P15735 (http://www.uniprot.org/uniprot/P15735)

Screening Location
Malvern, USA

Reference compound IC50 for PHKg2
Reference compound IC50 for PHKg2