Activity-based PI3K (p110a/p55g) lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.
Target Overview
CAT#
PI3K (p110a/p55g)
Enzyme
Human PI3K (p110a/p55g)
HGNC
PIK3CA, PIK3R3
Synonym
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, Phosphoinositide-3-kinase, regulatory subunit 3 (Gamma)
Scientific Information
Assay Properties
Substrates
PI(4,5)P2:PS, 10 µM
ATP, 10 µM
ATP, 10 µM
Reaction
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + ATP = 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| PI-103 | 1.7 |
| PIK-93 | 7.7 |
| GSK-A1 | 7.1 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound inhibition of PI3K (p110a/p55g)
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