PI3K (p110a(E545K)/p85a) Kinase Assay Service

Activity-based PI3K (p110a(E545K)/p85a) lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.

Target Overview

CAT#

PI3K (p110a(E545K)/p85a)

Enzyme

Human PI3K (p110a(E545K)/p85a)

HGNC

PIK3CA (E545K)-PIK3R1

Synonym

MGC142161, MGC142163, p110-alpha, PI3K, PI3K-alpha

Substrates

PI(4,5)P2:PS, 10 µM
ATP 10 µM

Assay Properties

Reaction

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + ATP = 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Compounds	IC50 (nM)
PI-103	3.3

Scientific Information

p110a: P42336 (http://www.uniprot.org/uniprot/P42336)P85a: P27986 (http://www.uniprot.org/uniprot/P27986)

Screening Location

Malvern, USA

Reference compound IC50 for PI3K (p110a(E545K)/p85a)