PI3Kb (p110b/p85b) Kinase Assay Service

Activity-based PI3Kb (p110b/p85b) lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.
Target Overview
CAT#
PI3Kb (p110b/p85b)
Enzyme
Human PI3Kb (p110b/p85b)
HGNC
PIK3CB-PIK3R2
Synonym
PIK3CB, PIK3C1
Scientific Information
p110b: P42338 (http://www.uniprot.org/uniprot/P42338)
p85b: O00459 (http://www.uniprot.org/uniprot/O00459)
Assay Properties
Substrates
PI(4,5)P2:PS, 10 µM
ATP, 10 µM
Reaction
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + ATP = 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Compounds IC50 (nM)
PI-103 6.8
PIK-93 139.4
GSK-A1 76
KU-55933 381.5
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound inhibition of PI3Kb (p110b/p85b)
Reference compound IC50 for PI3Kb (p110b/p85b)
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