Activity-based PI3KC3 lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.
Target Overview
CAT#
PI3KC3
Enzyme
Human PI3KC3
HGNC
PIK3C3
Synonym
MGC61518, PI3-kinase type 3, PI3K type 3, Vps34
Substrates
PI:PS, 50 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
1-phosphatidyl-1D-myo-inositol + ATP = 1-phosphatidyl-1D-myo-inositol 3-phosphate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| SAR405 | 11 |
| PIK-93 | 1,100 |
Scientific Information
Q8NEB9 (http://www.uniprot.org/uniprot/Q8NEB9)
Screening Location
Malvern, USA
Reference compound IC50 for PIK3C3
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