Activity-based PI3Kg (p110g) lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.
Target Overview
CAT#
PI3Kg (p110g)
Enzyme
Human PI3Kg (p110g)
HGNC
PIK3CG
Synonym
p120-PI3K, PI3CG, PI3K, PI3Kgamma, p110-gamma
Substrates
PI(4,5)P2:PS, 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + ATP = 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| PI-103 | 37.8 |
Scientific Information
p110g: P48736 (http://www.uniprot.org/uniprot/P48736)
Screening Location
Malvern, USA
Reference compound IC50 for PI3Kg (p110g)
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