Activity-based PI4Ka lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.
Target Overview
CAT#
PI4KA
Enzyme
Human PI4KA
HGNC
PI4KA
Synonym
PIK4CA
Substrates
PI, 100 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
1-phosphatidyl-1D-myo-inositol + ATP = 1-phosphatidyl-1D-myo-inositol 4-bisphosphate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| PIK-93 | 530 |
Scientific Information
P42356 (http://www.uniprot.org/uniprot/P42356)
Screening Location
Malvern, USA
Reference compound IC50 for PI4KA
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