Activity-based PI4Kb lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.
Target Overview
CAT#
PI4KB
Enzyme
Human PI4KB
HGNC
PI4KB
Synonym
PIK4CB, NPIK
Substrates
PI, 100 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
1-phosphatidyl-1D-myo-inositol + ATP = 1-phosphatidyl-1D-myo-inositol 4-bisphosphate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| PIK-93 | 5.3 |
Scientific Information
Q9UBF8 (http://www.uniprot.org/uniprot/Q9UBF8)
Screening Location
Malvern, USA
Reference compound IC50 for PI4KB
PDF