Activity-based PIK3CA E542K/PIK3R1 kinase assay for compound screening and profiling via ADP-Glo – assay technology
Target Overview
RBE Target ID
PIK3CA E542K/PIK3R1
Enzyme
Human PIK3CA E542K/PIK3R1
HGNC
PIK3CA, PIK3R1
Manning ID
N/A
Synonym
MGC142161, MGC142163, p110-alpha, PI3K, PI3K-alpha, PIK3CA, PI3K-alpha, PIK3CA/PIK3R1, PI3Ka (p110a/p85a)
UniProt
P42336 (PK3CA_HUMAN)
Kinase Type
Lipid kinase
Kinase Group
PI3K
Assay Properties
Assay Format
ADP-Glo
Substrates
PIP2:PS, ATP
Reaction
PIP2:PS + ATP = P-PIP2:PS + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Wortmannin | 18 |
| PI-103 | 37 |
| LY294002 | 4900 |
| PIK93 | 59 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | PIK3CA E542K/PIK3R1 |
| wt Profiler | No |
| IC50 wt Profiler | No |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | No |
| Product Number | N/A |
| Web Shop | Reaction Biology Online Shop |
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