Activity-based PIK3CB E633K/PIK3R1 kinase assay for compound screening and profiling via ADP-Glo – assay technology
Target Overview
RBE Target ID
PIK3CB E633K/PIK3R1
Enzyme
Human PIK3CB E633K/PIK3R1, mutant
HGNC
PIK3CB (E633K)-PIK3R1
Manning ID
N/A
Synonym
PIK3CB, P110BETA, PI3K, PI3KBETA, PI3K-beta, PIK3C1, PIK3CB E633K/PIK3R1
UniProt
P42338 (PK3CB_HUMAN)
Kinase Type
Lipid kinase
Kinase Group
PI3K
Assay Properties
Assay Format
ADP-Glo
Substrates
PIP2:PS, ATP
Reaction
PIP2:PS + ATP = P-PIP2:PS + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
Compounds | IC50 (nM) |
---|---|
Wortmannin | 310 |
LY294002 | 2600 |
PI-103 | 140 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
FreeChoice | PIK3CB E633K/PIK3R1 |
345 wt Profiler | No |
345 IC50 wt Profiler | No |
Mutant Profiler Addon | No |
Lipid Kinase Profiler Addon | No |
IC50 Profiler | No |
Recombinant Protein Availability
Active recombinant protein |
Yes; ProQinaseTM – PIK3CB E633K/PIK3R1 |
Product Number |
1531-1165-1 |
Web Shop | Reaction Biology Online Shop |
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